| 產(chǎn)品優(yōu)勢(shì): | ? 高特異性:高封閉Taq抗體和優(yōu)化的PCR緩沖液雙重作用大大降低了引物二聚體的產(chǎn)生。 ? 室溫穩(wěn)定:在儲(chǔ)存和運(yùn)輸過(guò)程中,性能不容易隨溫度變化而降低;完全室溫配制體系。 ? 高GC擴(kuò)增:優(yōu)化的緩沖液配方可高效擴(kuò)增GC 含量高達(dá) 80% 的模板,從而簡(jiǎn)化引物設(shè)計(jì)。 ? 寬線性范圍:可在8個(gè)模板梯度線性范圍內(nèi)展示很高的重復(fù)性。 |
| 描述: | LiQuant? Ultra Green qPCR Master Mix一款經(jīng)典定量 PCR 試劑盒,基于 SYBR Green I 染料法的 2×預(yù)混液,包含除引物和模板外的所有組分。本產(chǎn)品采用抗體法熱啟動(dòng)的 Taq 酶,可進(jìn)行高特異、高穩(wěn)定的定量 PCR 分析。該酶通過(guò)特殊配方優(yōu)化,適用于高 GC 含量模板的擴(kuò)增,可在 37℃ 下 7 天性能保持不變,具有很高的穩(wěn)定性,推薦室溫加樣操作。該試劑中已預(yù)混 ROX,可兼容大部分定量 PCR 儀。 |
| 組分: | 1. LiQuant? Ultra Green qPCR Master Mix: 5 ml |
| 存儲(chǔ)條件: | 2-8℃保存1年 |
| 文檔: | 說(shuō)明書(shū) MSDS |

Figure 1. Comparison of the specificity between LiQuant? Ultra and Brand N
Template DNA: Eight 10×Dilutes of the pET28a plasmid with Bacillus badius phenylalanine dehydrogenase gene.
Primer: Forward primer AGGAAGCCGATGTGTTCGTT Reverse primer TTCCGCTTGCTGGTACACTT
From the melting curve, it shows that LiQuant? Ultra exhibits a single peak under both low and high concentration templates, while under low concentration templates, Brand N exhibits non-specific amplification.

Figure 2. High stability verification
Template DNA: Eight 10×Dilutes of the pET28a plasmid with Bacillus badius phenylalanine dehydrogenase gene.
Primer: Forward primer AGGAAGCCGATGTGTTCGTT Reverse primer TTCCGCTTGCTGGTACACTT
From the amplification curve, it shows that the LiQuant? stored at 37 ℃ and at -20 ℃ have the same curve, and the Cq value is basically similar. From the standard curve, it shows that the PCR efficiency of LiQuant? at different stored temperatures are both at 95% -100%, and the R2 value is 0.999.