成功案例

Figure1. 與RIPA緩沖液相比，IntactProtein?裂解試劑盒在提取大尺寸蛋白質方面表現出優異的性能

A. 分別使用IntactProtein?裂解試劑盒和RIPA緩沖液裂解HeLa細胞，并使用mTOR（289 kDa）抗體進行蛋白免疫印跡，裂解物上樣量為50μg。
B. HT1080細胞裂解物（50 μg）在如圖所示處理的冰上裂解，并用抗SMRT（160，270 kDa）抗體印跡。注意，超聲將SMRT片段化為更小的肽。

Figure2. IntactProtein? 裂解試劑盒在從組織和細胞中提取蛋白質方面表現同樣出色

A. 使用 IntactProtein? 裂解試劑盒裂解 HeLa 細胞和小鼠大腦（一式兩份）。使用mTOR (289 kDa)抗體對50 pg總裂解物進行免疫印跡。
B. 用抗GAPDH (37 kDa)抗體對(A)中的相同裂解物進行印跡。 請注意，IntactProtein? 裂解試劑盒適用于從細胞和組織中提取大分子量和小分子量的蛋白質。

Figure3. IntactProtein? 裂解試劑盒能很好地保護蛋白質翻譯后修飾 (PTM)。

A-B. 使用 IntactProtein?裂解試劑盒對提取Hela細胞，Hela細胞無血清培養16小時后用胰島素(150 nM) 處理 5 分鐘，隨后使用IntactProtein? 裂解試劑盒提取蛋白。 分別使用p-MTOR (Ser2448) (A) 和 mTOR (B) 抗體對50 μg 完全產物進行蛋白免疫印跡。
C. 分別使用 RIPA 緩沖液或 IntactProtein? 裂解試劑盒裂解 HeLa 細胞。 使用抗 O-連接 B-N-乙酰葡糖胺 (O-GcNAc)（糖基化）抗體對細胞裂解物進行印跡。

發表文章

1. PPARγ Acetylation inAdipocytes Exacerbates BAT Whitening and Worsens Age-AssociatedMetabolic Dysfunction. 
發表期刊：Cell        產品：IntactProtein? Cell-Tissue Lysis Kit

2. Salidroside reducesneuropathology in Alzheimer's disease models by targeting NRF2/SIRT3pathway. 
發表期刊：Cell Biosci         產品：IntactProtein? Cell-Tissue Lysis Kit

3. Ventromedial hypothalamic OGT drives adipose tissue lipolysis and curbs obesity.  
發表期刊：Advanced Science        產品：IntactProtein? Cell-Tissue Lysis Kit

4. O-GlcNAcylation is a gatekeeper of porcine myogenesis.  
發表期刊：J Anim Sci        產品：IntactProtein? Cell-Tissue Lysis Kit

5. PPARγAcetylation Orchestrates Adipose Plasticity and Metabolic Rhythms. 
發表期刊：AdvSci (Weinh)        產品：IntactProtein? Cell-Tissue Lysis Kit

客戶評價

"I was always having trouble dealing with extraction of large-sized proteins such as mTOR (MW 289) from cultured cells. My problem was that without sonication, the extraction efficiency of large proteins was low; with sonication, the large-sized proteins were fragmented into smaller-sized peptides. This dilemma was finally solved by using IntactProtein? Cell-Tissue Lysis Kit. With this kit, I obtained excellent results without losing phosphorylation signals. Moreover, this kit was easy to use by simply mixing reagent A and B and lysing the cells for 15 min on ice."

"We have tested your IntactProtein? Cell-Tissue Lysis Kit in liver, adipose tissue, and cultured cells. It worked really well! Extracting proteins from fat is always a headache. Your buffer gave us high protein yields and sharp protein bands. We’d like to switch to your buffer"